Analytical Testing Facility
One stop solution for your Innovation
Analytical Testing Facility
State of the art licensed commercial grade analytical test facility.
Cell Culture Facility
Expert consultation for cell culture needs and experimental design.
Bioprocess Lab
Utilizes living cells or their cellular components, viruses or an entire organism.
Common Instrumentation
Enriching the resources on a shared basis for promoting research activities.
Industry Leading Equipments
KIIT-TBI Analytical Testing Facility (KIIT-TBI ATF) delivers a one solution for all kinds of analytical services under one roof for manufacturers, exporters, importers, rowers, academicians, innovators and researchers with the trust of compliance of all National and International regulatory bodies and agencies.
Equipped with
Analytical Testing Facility
Agilent 6420 Triple Quadrupole LC/MS System
- Ionisation Sources: ESI, APCI, APPI ,
- Detector: Electron multiplier
- Mass accuracy within 1 ppm; m/z (z=1) less than 500
- Mass Analyzer: Quadrupole Time Of Flight (QTOF)
- MS/MS accuracy within 2 ppm
- S/N ratio of 450:1
- Resolution of 20,000 at m/z 1522
- High Femtogram level sensitivity, I pg at m/z 609
- Dynamic range of up to five orders
- Ability to scan multiple precursor ions ~50
- Rapid polarity switching of 1.5 sec
- Scan rates of 50 Hz
- Positive to negative ion modes
- Mass accuracy temperature stability, MS mode: maintaining 1 ppm mass accuracy
- Mass accuracy within 2 ppm gives you complete confidence in your analysis.
- Powerful Agilent Jet Stream source enables you to uncover low level components.
- Wide in-spectrum dynamic range facilitates simultaneous measurement of minor components in the presence of intense background interferences.
- The latest developments in Agilent Infinity II UHPLC, 2D-LC, SFC, and even GC-APCI chromatographic interfaces give you blazing fast acquisition speed.
- Powerful MassHunter software provides the latest software capabilities for profiling, characterizing, identifying, and quantifying compounds in complex mixtures via high-definition MS and MS/MS.
- Untargeted screening and quantitation using Agilent’s All Ions MS/MS technique.
Agilent 6530 Q-TOF LC/MS System
- Mass Analyzer: Triple Quadruple
- Ionisation Sources: ESI, CI, AJS
- Detector: Electron multiplier
- Mass accuracy within 1 ppm; 0.1 Da from 5-1000 m/z
- MS/MS accuracy :0.01% from 1000-2000 m/z
- S/N ratio of 600:1
- Resolution of 0.7 Da at m/z 1000
- High Femtogram level sensitivity, I pg CAP at m/z 321
- Dynamic range of up to Six orders
- Ability to scan multiple precursor ions ~60
- Rapid polarity switching of 30 ms
- Scan rates of 50 Hz
- Positive to negative ion modes
- Mass accuracy temperature stability, MS mode :maintain 1 ppm mass accuracy
- Powerful Mass Hunter software provides the latest software capabilities for profiling, characterizing, identifying, and quantifying compounds in complex mixtures via high-definition MS and MS/MS.
- Untargeted screening and quantitation using Agilent’s All Ions MS/MS technique.
- Wide in-spectrum dynamic range facilitates simultaneous measurement of minor components in the presence of intense background interferences.
GC MS ION TRAP with Headspace Sampler (7890B, 7697A, 240-GC MS ION TRAP with Headspace)
- Ion preparation techniques can be performed after ionization but before ion analysis. These include
- Selected Ion Storage (SIS)
- Tandem Mass Spectrometry
- MS/MS
- Multiple Reaction Monitoring (MRM)
- Mass range :50-500 m/z
- IDL sensitivity : 100 fg OFN 272-222 m/z
- External Ionization is one of three configurations of the 240 GC/MS system. It can be used with electron impact ionization (EI), positive chemical ionization (PCI), and negative chemical ionization (NCI).
- Single extraction mode with overlapping of up to 12 vials for maximized sample throughput while maintaining constant heating time for each vial
- Multiple headspace extraction (MHE) mode with up to 100 extractions per vial
- Multiple headspace concentration (MHC) mode with up to 100 extractions from a single vial followed by one GC start to maximize sensitivity
- Method development mode used to optimize headspace extraction by incrementing one of the following parameters: equilibration time, oven temperature, or vial shaking.
Agilent GC FID with MS Detector 7890 B ,5977 B
- Ion preparation techniques can be performed after ionization but before ion analysis. These include
- Selected Ion Storage (SIS)
- Tandem Mass Spectrometry
- MS/MS
- Multiple Reaction Monitoring (MRM)
- Mass range :50-500 m/z
- IDL sensitivity : 100 fg OFN 272-222 m/z
- External Ionization is one of three configurations of the 240 GC/MS system. It can be used with electron impact ionization (EI), positive chemical ionization (PCI), and negative chemical ionization (NCI).
- Single extraction mode with overlapping of up to 12 vials for maximized sample throughput while maintaining constant heating time for each vial
- Multiple headspace extraction (MHE) mode with up to 100 extractions per vial
- Multiple headspace concentration (MHC) mode with up to 100 extractions from a single vial followed by one GC start to maximize sensitivity
- Method development mode used to optimize headspace extraction by incrementing one of the following parameters: equilibration time, oven temperature, or vial shaking.
Cell Culture Facility
Eppendorf Galaxy 170 R CO Incubator
Display readout: Temperature and CO2 level
high temperature disinfection
with humidity monitoring package
Tradename
Eppendorf® CO17214015
Parameter
0.2-20% (CO2 range)
ambient +4 to 50 °C temprature range
W × D × H
70.8 cm × 71.1 cm × 84.5 cm
Weight
89.9 kg
Capacity
170 L
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Beckman Coulter Allegra X-30R Refrigerated Centrifuge
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NuAire biosafety cabinet
| AIR CLEANLINESS (ISO 14644) | ISO-Class-5 |
|---|---|
| AIRFLOW MONITOR | AirflowSensorPressureGrid |
| CABINET CONSTRUCTION | All welded stainless steel 16/18 GA, Type 304 pressure tight design |
| CONTROL SYSTEM | TouchLink |
| DOWNFLOW FPM (M/S) | 60 fpm (0.30 m/s) |
| PROTECTION | [, Product, Personnel, Environmental] |
| WORK ACCESS OPENING(S) | 8-inches (203 mm) |
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Cell Bioprocessing
Eppendorf BioFlo/CelliGen 115 Bioreactor 7.5 Litre and 14.0 Litre
Display readout: Temperature and CO2 level
high temperature disinfection
with humidity monitoring package
Tradename
Eppendorf® CO17214015
Parameter
0.2-20% (CO2 range)
ambient +4 to 50 °C temprature range
W × D × H
70.8 cm × 71.1 cm × 84.5 cm
Weight
89.9 kg
Capacity
170 L
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AKTA Pure: Protein Purification System
Display readout: Temperature and CO2 level
high temperature disinfection
with humidity monitoring package
Tradename
Eppendorf® CO17214015
Parameter
0.2-20% (CO2 range)
ambient +4 to 50 °C temprature range
W × D × H
70.8 cm × 71.1 cm × 84.5 cm
Weight
89.9 kg
Capacity
170 L
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Refrigerated Centrifuge Sigma 4-16KS
Display readout: Temperature and CO2 level
high temperature disinfection
with humidity monitoring package
Tradename
Eppendorf® CO17214015
Parameter
0.2-20% (CO2 range)
ambient +4 to 50 °C temprature range
W × D × H
70.8 cm × 71.1 cm × 84.5 cm
Weight
89.9 kg
Capacity
170 L
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Common Instrumentation
Quant Studio 5 RT-PCR
Sample capacity (wells) | 96 |
Reaction volume | 96-well 0.2 mL block: 10-100 μL |
Excitation source | Bright white LED |
Filter or color combinations | 6 decoupled filters, CMOS camera |
Excitation/detection range | 450-680 nm/500-730 nm |
Multiplexing | 5-plex + 1 passive reference, 6-plex with no passive reference |
Maximum block ramp rate | 6.5°C/sec |
Average sample ramp rate | 3.66°C/sec |
Temperature uniformity | 0.4°C |
- Results you can trust—detect differences in target quantity as small as 1.5-fold in singleplex reactions and obtain 10 logarithmic units of linear dynamic range
- Simple, powerful software—allows users to set up a run, lay out assays, control the instrument, and conduct plate analysis within a single, easy-to-use software interface
- Proven performance—over 10 years of clinical real-time PCR instrument manufacturing
- Designed with the clinic in mind—interactive diagnostic instrument with short run times, minimal maintenance, and use of existing plastic consumables
- Enhanced security—auditing and e-signature functionalities that enable you to comply with regulations
- Peace of mind—IVD test menu allows only authorized IVD tests to be run through IVD mode, helping to reduce the risk of unauthorized use and accidental or intentional misuse
- Flexibility you need—diagnose or develop, the choice is yours with software options that guide you through every step of test development* and in vitro diagnostic (IVD) modes
- Maximize benchtop space—compact instrument
- Superior support—support available globally by highly skilled, customer-focused staff
- QuantStudio performance—reliability, sensitivity, and accuracy you expect coupled with an intuitive and simple-to-use interface that allows users of any experience level to operate the system with ease
Gradient PCR Nexus Gradient and Vapo Protect
Unit can only be controlled1 | – |
Thermoblock | Aluminum |
Sample capacity | 96 × 0.2 mL PCR tubes, 1 PCR plate (96 wells) or up to 38 × 0.5 mL Eppendorf PCR tubes |
Heating technology | Peltier elements, Triple Circuit Technology |
Temperature control mode | Fast, Standard, Safe |
Temperature control range of the block | 4 – 99 °C |
Gradient range | 1 – 20 °C |
Intuitive graphic programming | ￭ |
Gradient temperature control range | 30 – 99 °C |
Block homogeneity 20 – 72 °C | ≤ ±0.3 °C |
Control accuracy | ±0.2 °C |
E-mail Notification | ￭ |
Roch Mechatronics are Manufacturer supplier of Gradient PCR , Laboratory PCR, PCR Detection System, PCR Thermal Cycler, Thermal Cycler PCR, PCR DNA Engine, Shaker. when Specific DNA fragment are to be Amplified and Single or multiple copies are to be produced with desired Temperature Ramp and soak Gradient,
- Roch Mechatronics Gradient PCR, Windows operating system.
- Automatic restart after power failure.
- The actual and target temperatures can be viewed and displayed graphically in real time.
- Built – in 11 standard program file template, can quickly edit the required file.
- Compatible with mouse, keyboard, capable to transfer data and perform software updates via USB Drive.
- Replaceable module, dual block module can simultaneously run two different PCR programs.
- Advance file management :- Roch Mechatronics PCR has inbuilt advance scheduling feature , data storage run edit, save the suggested protocol.
- Security features: – Password protected folders, option al login and secured mode for highly regulated environment.
- Calibration :- Validation and calibration facility available in system.
- Power saves :- Standby function.
- Feature of instant incubation to keep samples at constant temp. For ligation and restriction digests.
- Compatible with all kind of plastic consumables and reagents especially reusable sealing mats.
Biorad BioLogic DuoFlow Protein Purification System
The Innova 42R incubated and refrigerated shakers are our most versatile incubator shakers. The programmable Innova 42 and 42R provide exceptional convenience allowing you to simultaneously shake and static incubate cultures under the same conditions. Use on or under the bench, or double stacked for space savings. An optional culture drawer, gassing manifold, remote alarm, and humidity monitor are available for both models; a photosynthetic light bank and UV germicidal light are available for the 42R.
BioLogic DuoFlow software is easy and intuitive, making it a powerful program for both novice and experienced chromatographers. The software walks you step by step through simple protocols to create and run a method. Setup of new hardware and methods is easy, and the software offers many post-run options.
Experiments are set up through an easy-to-follow wizard format. The software provides functions such as:
- Scouting wizard — provides simplified setup of scouting experiments. Scouting optimizes purification of a target molecule by changing a variable incrementally with each scouting run
- Method templates — allow easy method creation with predefined chromatography method templates for all commonly used chromatography experiments, including affinity, ion exchange, chromatofocusing, hydrophobic interaction, hydroxyapatite, and size exclusion (gel filtration) chromatography, as well as more advanced experiments
- Buffer blending — enables, in combination with the BioLogic Maximizer valve system, automatic blending of up to 4 stock solutions, allowing pH and salt concentration control within a specified run
BioLogic DuoFlow software provides peak recovery control and data review with the following features:
- Trace Compare function — permits overlay of any number of different chromatograms for comparison of runs made under different conditions, such as pH, flow rate, buffer gradients, etc.
- Fraction identification — provides BioFrac fraction collector numbering schemes that number tubes by collection order or by rack grid number; this is especially useful for microplate collection.
- Threshold collection — allows collection of fractions when a detector signal is above or below a defined threshold; this is especially useful for desalting and chromatofocusing applications
- Tagging of peaks — labels peaks with name, retention time, absorbance units (UV trace), pH, or conductivity
- Selection of an activity trace — permits data collected by an offline method to be included with the BioLogic DuoFlow run data; a histogram of the offline data can overlay the chromatogram peaks
Eppendorf Innova 42R Orbital shaker incubator
Specifications | Innova® 42R, orbit diameter 1.9 cm (3/4 in) |
External depth (with lid/door open) | 131 cm (51.6 in) |
Timer | 0.1 – 99.9 h, continuous |
Power supply | 230 V, 50/60 Hz |
Dimensions (W × D × H) | 63.5 × 74.5 × 81.8 cm / 25 × 29.3 × 32.8 in |
Weight w/o accessories | 121 kg / 267 lb |
Temperature range | 20 °C below ambient to 80 °C |
Audible and visual alarms | yes |
Temperature uniformity | ±0.25 °C at 37 °C |
Available program modes | • Constant speed and temperature |
• Programmable multi-steps | |
• RS-232 communication port | |
Drive mechanism | triple-eccentric counterbalanced drive. |
- High capacity in a small footprint: for example, 4x 5 L or 2x 6 L flasks on universal platform; device footprint 4731 cm2 /733 in2 (63.5 cm/25 in width, 74.5 cm/29.3 in depth)
- Ergonomical and safe interface handling: Top mounted controls and display make bending down unnecessary during handling, avoids accidental parameter changes, and can be reached easily even in a double stack
- Easy to clean: Spill diverter and drainable built-in spill pan with rounded corners protect the drive from accidental spills (can be used as a water reservoir to reduce sample evaporation); airflow UV-germicidal light optional
- Lab position flexibility: Lab position on or under bench, 2x stackable
- Parallel static cultivation: One height adjustable static shelf included, optional culture drawer and additional shelves available
- Sample evaporation protection: Bottom spill pan usable as water reservoir; optional culture drawer, for Agar plates, for example
- Visual sample control: Large viewing window and internal light provide easy viewing of samples without the need to open the door
- Reliable 24/7 with high loads: Proven and tested Innova triple eccentric counterbalanced drive (three weight-supporting shafts), max. load 15 kg
- Programmability: Continuous, timed, or multi-step programming (15 steps), store up to four programs
- Options and Accessories: Orbit 19 or 25 mm, photosynthetic light bank, UV germicidal light, culture drawer for evaporation protection of Agar plates, additional static shelves (one included), humidity monitoring, remote monitoring with VisioNize® Lab Suite and more.