Molecular Diagnostics & Immunoassays
The Genetic and Cellular Portrait: Advanced Diagnostic Platforms
Conducted at the Thermo Fisher Scientific Centre of Excellence, this two-day module bridges the gap between nucleic acid amplification and high-end single-cell phenotyping.
Day 1: Genomics & Molecular Diagnostics
Module 1
Principles of Precision Diagnostics
Fundamentals of PCR, RT-PCR, and the science of primer/probe design.
Module 2
DNA/RNA Analysis
Fluorescence-based quantification (Qubit) and setting up diagnostic workflows on thermal cyclers.
Module 3
Data Interpretation
Evaluating amplification curves.
Day 2: Immunoassays & Flow Cytometry
Module 4
Advanced Cell Analysis
Principles of acoustic-assisted fluidics and fluorophore selection for immunophenotyping.
Module 5
Flow Cytometry Workflow
Operating the Attune™ NxT for cell cycle analysis and rare cell detection.
Module 6
Advanced Gating
Transforming raw data into multi-parametric histograms and troubleshooting spectral overlap.
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